Oncostatin M promotes the differentiation of murine induced pluripotent stem cells into cardiomyocytes
- VernacularTitle:抑瘤素M促进小鼠诱导多能干细胞向心肌分化
- Author:
Ji WANG
1
;
qin Yu LU
;
Xin XU
;
juan Zhao JI
Author Information
1. 甘肃中医药大学附属医院 心血管科
- Keywords:
oncostatin M;
miPSCs;
myocardial cell;
differentiation
- From:
Basic & Clinical Medicine
2017;37(10):1401-1406
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of oncostatin M(OSM) on differentiation of murine induced pluri-potent stem cells(miPSCs) into cardiomyocytes in vitro. Methods Using the embryoid bodies differentiation meth-od,OSM was added during early-phase and midphase(day 0-6) of miPSCs differentiation for 15 days.Semi-quan-titative RT-PCR,Western blot and immunofluorescence staining were used to detect the expression levels of myo-cardial cells-specific markers. Results The best concentration of OSM to induce the cardiac differentiation of miP-SCs was 20 pmol/L and cardiac troponin-T(cTnT) was expressed 7-fold higher in OSM-treated compared with vehi-cle control-treated embryoid bodies at day 15 post-differentiation. Cardiac-specific gene and protein expression of cTnT and Gata4 demonstrated that the OSM-treated embryoid bodies expressed cardiac-specific gene(Tnnt2, Nkx2.5,MLC2a,MLC2v,GATA4 and GATA6) and protein (cTnI,Gata4) at levels that were higher than those of the vehicle-control group after induced for 15 days. Furthermore, Immunostaining of the cardiac-specific protein markers cTnT showed that the miPSCs expressed cTnT across all groups, and exhibited a markedly increased of cTnT-positive cells in OSM-treated cells. Conclusions OSM promotes the differentiation of murine induced pluripo-tent stem cells into cardiomyocytes, and it could serve as a valuable strategy for myocardiac differentiation of miPSCs in vitro.
