Analysis on interference of blood urine on UF-1000i automatic urinary sediment analyzer
10.7687/j.issn1003-8868.2017.07.101
- VernacularTitle:UF-1000i全自动尿有形成分分析仪对血尿检测的携带污染分析
- Author:
wei Dan WANG
1
;
Jun NI
Author Information
1. 南京大学医学院附属鼓楼医院检验科
- Keywords:
automatic urinary sediment analyzer;
blood urine;
detection;
carryover;
microscopy
- From:
Chinese Medical Equipment Journal
2017;38(7):101-103,114
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the interference of blood urine on red cell detection of subsequent adjacent specimen on Sysmex UF-1000i flow cytometer urine sediment analyzer and to propose some countermeasures.Methods Totally 60 copies of fresh urine specimens having the results in normal range were chosen,and then each copy was divided into three pieces.All the pieces of specimens were put on the tube rack between the low-,medium-and high-concentration blood urine,and the urine was in front of the specimens.UF-1000i automatic urinary sediment analyzer was used for detection under auto sampling mode,and paired t-test was carried out for the results before and after interferences by concentrations of blood urine.The carryover was measured for the specimens and blood urine,and the specimens underwent microscopy.Results The RBC results had no significant difference before and after the interference by low-concentration blood urine (P＞0.05),while had statistical differences before and after the interferences by medium-and high-concentration blood urine (P＜0.01).There was also obvious difference between the RBC results interfered by medium-concentration blood urine and by highconcentration one.There were no significant differences between the results of WBC,epithelial cell and cast before and after the interferences (P ＞0.05).The urinary sediment microscopy results were within the normal range,and the highconcentration blood urine had the carryover gain the maximum value.Conclusion UF-1000i automatic urinary sediment analyzer has the RBC result higher than the actual one after the interference,and the interference deteriorates with the increased concentration of blood urine.The blood urine specimen has to be tested separately,and deep cleaning has to be performed for the analyzer.The test by the analyzer had better be combined with microscopy to avoid carryover.
