Four-week histologic evaluation of grafted calvarial defects with adjunctive hyperbaric oxygen therapy in rats.
10.5051/jpis.2016.46.4.244
- Author:
Hyeyoon CHANG
1
;
Seo Eun OH
;
Seunghan OH
;
Kyung Seok HU
;
Sungtae KIM
Author Information
1. Department of Periodontology, Dental Research Institute, Seoul National University School of Dentistry, Seoul, Korea. kst72@snu.ac.kr
- Publication Type:Original Article
- Keywords:
Biphasic calcium phosphate;
BMP-2;
Bone substitute;
EGCG;
Hyperbaric oxygen therapy
- MeSH:
Animals;
Bone Substitutes;
Calcium;
Humans;
Hyperbaric Oxygenation*;
Male;
Osteogenesis;
Parietal Bone;
Rats*;
Rats, Sprague-Dawley;
Transplants*
- From:Journal of Periodontal & Implant Science
2016;46(4):244-253
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: The aim of this study was to characterize the healing in the grafted calvarial defects of rats after adjunctive hyperbaric oxygen therapy. METHODS: Twenty-eight male Sprague-Dawley rats (body weight, 250–300 g) were randomly divided into two treatment groups: with hyperbaric oxygen therapy (HBO; n=14) and without HBO (NHBO; n=14). Each group was further subdivided according to the bone substitute applied: biphasic calcium phosphate (BCP; n=7) and surface-modified BCP (mBCP; n=7). The mBCP comprised BCP coated with Escherichia-coli-derived recombinant human bone morphogenetic protein-2 (ErhBMP-2) and epigallocatechin-3-gallate (EGCG). Two symmetrical circular defects (6-mm diameter) were created in the right and left parietal bones of each animal. One defect was assigned as a control defect and received no bone substitute, while the other defect was filled with either BCP or mBCP. The animals were allowed to heal for 4 weeks, during which those in the HBO group underwent 5 sessions of HBO. At 4 weeks, the animals were sacrificed, and the defects were harvested for histologic and histomorphometric analysis. RESULTS: Well-maintained space was found in the grafted groups. Woven bone connected to and away from the defect margin was formed. More angiogenesis was found with HBO and EGCG/BMP-2 (P<0.05). None of the defects achieved complete defect closure. Increased new bone formation with HBO or EGCG/BMP-2 was evident in histologic evaluation, but it did not reach statistical significance in histometric analysis. A synergic effect between HBO and EGCG/BMP-2 was not found. CONCLUSIONS: Within the limitations of this study, the present findings indicate that adjunctive HBO and EGCG/BMP-2 could be beneficial for new bone formation in rat calvarial defects.
