Serotype distribution of Salmonella tcpS gene and its application in identification of specific serovars
10.3969/j.issn.1002-2694.2017.09.001
- VernacularTitle:沙门菌tcpS基因的血清型分布及其在特定血清型鉴定中的应用
- Author:
Dan XIONG
1
;
Li SONG
;
Jing TAO
;
juan Hui ZHENG
;
hao Zi ZHOU
;
ming Zhi PAN
;
an Xin JIAO
Author Information
1. 江苏省人兽共患病学重点实验室/江苏省动物重要疫病与人兽共患病防控协同创新中心/农业与农产品安全国际合作联合实验室/农业部农产品质量安全生物性危害因子(动物源)控制重点实验室
- Keywords:
Salmonella;
tcpS gene;
PCR;
serotype
- From:
Chinese Journal of Zoonoses
2017;33(9):757-762
- CountryChina
- Language:Chinese
-
Abstract:
A tcpS-based PCR method was established to simultaneously screen Salmonella enterica serovars Enteritidis,Pullorum/Gallinarum,and Dublin.The developed PCR method provides laboratorial support as a convenient and rapid approach for epidemiological investigation,and tcpS can be a potential candidate gene for the development of PCR-based Salmonella identification.The serotype distribution of Salmonella tcpS gene was analyzed by bioinformatic approach.The specificity and sensitivity of the PCR method were determined based on 27 different Salmonella serovars and 10 non-Salmonella strains.The PCR method was applied to clinical Salmonella isolates from one pig farm (48 isolates),one chicken farm (22 isolates) and one cattle farm (11 isolates) from Jiangsu Province.In silico analysis showed that tcpS existed only in Salmonella Enteritidis,Pullorum/Gallinarum,and Dublin.The developed PCR method had potent specificity and sensitivity,and could screen the three specific Salmonella serovars accurately.The coincidence rate of the clinical sample detection was up to 100％.The tcpS-based PCR detection method could screen Salmonella Enteritidis,Pullorum/Gallinarum,and Dublin accurately,and could be an assistant method to the traditional serotyping method.Furthermore,the novel tcpS gene can be a potent gene candidate for the development of PCR method for the identification of Salmonella serovars.
