Comparison and evaluation different methods for detecting metallo-β-lactamase
10.3969/j.issn.1673-4130.2017.18.009
- VernacularTitle:不同金属β-内酰胺酶检测方法的比较与评价
- Author:
Shulong ZHAO
1
;
Fei JIANG
;
Xiaojie ZHAO
;
Haiquan KANG
;
Lihua DENG
;
Bing GU
;
Ping MA
;
Hongyan HU
Author Information
1. 徐州医科大学附属医院检验科
- Keywords:
metallo-β-1actamase;
multiplex PCR;
bacterial resistance
- From:
International Journal of Laboratory Medicine
2017;38(18):2523-2525
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the applicationof the double-disk synergy test(DDST) and combined disk test(CDT) in clini cal metal enzyme phenotype deteetion.To evaluate the value of multiplex PCR in detecting the metallo-β-1actamase in clinical.Methods 56 strains of metallo-β-1actamase-positive strains were identified [NDM-1(n=9),VIM(n=32),IMP(n=15)]for appraise the two methods.By optimizing the design of PCR primers,3 pairs of primers were designed and detected (IMP,VIM,NDM-1) in one tube for evaluate the method.Results The DDST was 80.36％(45/56),and 100.00％(56/56) in the CDT.The accuracy of multiplex PCR was 100.00 ％ (56/56),and the size of the amplified fragment was used to distinguish three types of metallo-β-lactamase.Conclusion The CDT is more suitable for clinical application than DDST.Multiplex PCR has the characteristics of simple,rapid and accurate in detection of metallo-β-1actamase.It is suitable for daily use of clinical microbiological laboratory,which will help the clinical timely and effective administration.
