Effects of oxygen at varied concentrations on in vitro maturation of human immature oocytes and early-stage development of embryos
10.3969/j.issn.1006-5725.2017.20.016
- VernacularTitle:不同浓度氧气对人未成熟卵子体外成熟和早期胚胎发育的影响
- Author:
Yang LUO
1
;
Fang PENG
;
Hongzhi DU
;
Lei LI
Author Information
1. 广东省生殖医学重点实验室
- Keywords:
oxygen concentration;
immature oocyte;
in vitro maturation
- From:
The Journal of Practical Medicine
2017;33(20):3394-3397
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the effects of oxygen at varied concentrations on in vitro maturation (IVM)of oocytes,and subsequent fertilization,early-stage development of embryos by collecting the human imma-ture oocytes from assisted reproduction treatment. Methods Immature oocytes were randomly allocated to be cul-tured with oxygen at a lower or higher concentration. Intracytoplasmic sperm injection of mature oocytes after IVM , the rates of maturation,fertilization,embryo cleavage and high quality embryo were investigated. Results The GV oocytes cultured with oxygen at the lower concentration yielded higher maturation and fertilization rates than those at the higher concentration(P<0.05). There were no significant differences in the rates of embryo cleavage and high quality embryo between two groups(P > 0.05). For MI oocytes,the maturation rate of oocytes cultured with oxygen at the lower concentration was higher as compared to that at the higher concertration (P < 0.05). There were no significant differences in the rates of fertilization ,embryo cleavage and high quality embryo between the two groups(P > 0.05). Conclusions Oxygen at a lower concentration is beneficial to IVM of human imma-ture oocytes and it also improves the fertilization of GV oocytes after IVM. Oxygen at a lower concentration has no beneficial effect on the embryo cleavage rate and high quality embryo rate.
