Effects of Allicin on Transient Outward Potassium Current in Rat Atrial Myocytes
10.3870/j.issn.1004-0781.2017.11.004
- VernacularTitle:大蒜素对大鼠心房肌细胞瞬时外向钾电流的影响
- Author:
Chen CHEN
1
;
Rui WANG
;
Zhongqi CAI
;
Yi YANG
;
Kun LIN
;
Yuqi LIU
;
Jie YANG
;
Yang LI
Author Information
1. 首都医科大学附属北京安贞医院心内科
- Keywords:
Allicin;
Atrial myocytes;
Transient outward potassium current;
Patch clamp
- From:
Herald of Medicine
2017;36(11):1231-1235
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of allicin ( All) on potassium current in single atrial myocytes in rats. Methods Isolated rat atrial myocytes were isolated by perfusion and administered by extracellular perfusion method.Whole cell patch clamp technique was used to record transient outward potassium current ( Ito ) in atrial myocyte. Results In presence of 30 μmol·L-1 of All,the peak current of Ito was significantly reduced from (20.5±2.2) pA/pF to (11.3±2.1) pA/pF at+50 mV of test potential (P<0.01,n=12).This effect of All showed voltage- and concentration-dependence with IC50 of (19.0±2.5)μmol·L-1 .The steady-state activation curve of Ito was shifted to more positive potential and recovery time from inactivation was prolonged.In addition, they fail to find any effect of All on the steady-state inactivation and closed-state inactivation of Ito. Conclusion All can inhibit Ito by slowing down the process of activation and recovery from inactivation of channel.
