Effect of Dexmedetomidine Hydrochloride Preconditioning on Renal Function in Rats After Renal Ischemia Reperfusion Under High Glucose Condition
10.3870/j.issn.1004-0781.2017.11.005
- VernacularTitle:盐酸右美托咪定预处理对高糖状态下大鼠肾缺血-再灌注后肾功能的影响
- Author:
Wenwei GAO
1
;
Huaxin WANG
;
Bo ZHAO
;
Jiabao HOU
;
Xiaojing WU
Author Information
1. 武汉大学人民医院 重症医学科
- Keywords:
Dexmedetomidine;
hydrochloride;
High glucose;
Renal function;
Apoptosis
- From:
Herald of Medicine
2017;36(11):1236-1239
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of dexmedetomidine hydrochloride ( Dex ) preconditioning on renal function in rats after renal ischemia reperfusion injury under high glucose condition. Methods SD rats were randomly divided into 6 groups:NG-Sham operated group,NG-I/R group, NG-Dex group,HG-Sham operated group,HG-I/R group,HG-Dex group. Renal ischemia reperfusion model was established except Sham groups. Dex 50 μg·kg-1 was injected intraperitoneally 30 min before ischemia in the Dex preconditioning group,25% glucose 3 g·kg-1 was given intraperitoneally before the renal ischemia reperfusion model was established in high glucose groups. Blood glucose and renal function of each group were detected . Renal pathologic changes were observed with hematoxylin-eosin staining. Apoptosis of renal tissue was detected by TUNEL method. Results BUN,Cr and apoptosis rate in NG-I/R group were higher than those in NG-Sham operated group ( P<0.05);BUN,Cr and apoptosis rate in NG-Dex group were lower than those in NG-I/R group ( P<0.05);BUN,Cr and apoptosis rate in HG-I/R group and HG-Dex group were higher than those in NG-I/R group and NG-Dex group,respectively (P<0.05); However,there was no significant difference between HG-I/R group and HG-Dex group ( P>0.05) . Conclusion Dex has a protective effect on renal function after renal ischemia reperfusion, but this effect is inhibited in high glucose condition, which may relate to the increasing of kidney cells apoptosis.
