Effects of GBE50 on Hemodynamics and TNF-α Pathway in Myocardial Tissue of Rats with Acute Blood Stasis Syndrome
10.3969/j.issn.1005-5304.2017.11.009
- VernacularTitle:银杏酮酯对急性血瘀证大鼠血流动力学及心肌组织肿瘤坏死因子-α通路的影响
- Author:
Li JIANG
1
;
ping Zi YANG
;
min Yi BAO
Author Information
1. 上海中医药大学基础医学院
- Keywords:
Ginkgo biloba extract 50;
blood stasis syndrome;
hemodynamics;
TNF-α;
TNF receptor;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2017;24(11):34-38
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of Ginkgo biloba extract 50 (GBE50) on hemodynamics and myocardial tissue TNF-α pathway in rats with acute blood stasis syndrome; To discuss its mechanism of action. Methods A model of acute blood stasis syndrome was established by subcutaneous injection of epinephrine hydrochloride and ice bath. Male SD rats were divided randomly into normal group, model group and GBE50 group. After medicine was administrated by gavage for 11 days, the acute blood stasis model was established. The hemodynamic changes were observed in the next day. The expressions of TNF-α, TNFR1 and TNFR2 mRNA were detected by RT-PCR. The expressions of TNF-α, TNFR1 and TNFR2 protein were tested by radioimmunoassay and immunohistochemistry. Results The hemodynamics of the model group was lower than that of the normal group. Compared with model group, GBE50 could increase mLVSP, DP and decrease t-dp/dtmax(P<0.05). The protein expressions of TNF-α, TNFR1, and TNFR2 in GBE50 group were higher than those in model group, and TNFR2 mRNA expression increased significantly in GBE50 group (P<0.05). Conclusion GBE50 can ameliorate the decline of hemodynamics of rats with acute blood stasis syndrome, and regulate the myocardial TNF-α pathway. Increasing the expression of protective receptor TNFR2 may be one of the mechanisms of protecting cardiac function.
