Effects of Moxibustion at Different Temperatures on Blood Lipids and TRPV1 mRNA in Dorsal Root Ganglion with Hyperlipidemia Rats
10.3969/j.issn.1005-5304.2017.11.011
- VernacularTitle:不同温度艾灸对高脂血症大鼠血脂及背根神经节瞬时感受器电位香草酸受体1mRNA表达的影响
- Author:
ying Gui WANG
1
;
shuai Yao WANG
;
yun Jian GAO
;
fang Fang SU
;
yang Ruo CHEN
Author Information
1. 东南大学附属中大医院
- Keywords:
moxibustion temperature;
TRPV1;
blood lipid;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2017;24(11):44-47
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the effects of moxibustion at different temperatures on blood lipid and TRPV1 in dorsal root ganglion with hyperlipidemia rats; To verify the correlation between efficacy of adjusting fat of moxibustion with activating of TRPV1. Methods The rat model of hyperlipidemia was made by high fat diet. 60 SD mice were randomly divided into four groups: control group, model control group, the 38 ℃ moxibustion group and the 45 ℃ moxibustion group, with 15 mice in each group. Acupoints Shenque and Zusanli were chosen under moxibustion for 10 minutes each time, once another day, for 4 weeks, in the 38 ℃ moxibustion group and the 45 ℃moxibustion group. Blood was taken after intervention, and blood TC, TG, LDL-C and HDL-C of the mice were detected by oxidase method; the dorsal root ganglion was taken to detect the expression of TRPV1 mRNA by real-time fluorescence quantitative PCR. Results Compared with model group, blood lipid indexes in moxibustion groups had different changes, with statistical significance compared with 45 ℃ moxibustion group (P<0.05, P<0.01), and there was statistical significance between 38 ℃ moxibustion group and 45 ℃ moxibustion group (P<0.01); there was statistical significance in TRPV1 mRNA of dorsal root ganglion among 45 ℃ moxibustion group and other three groups (P<0.01). Conclusion The correlation between efficacy of adjusting fat of moxibustion with activating of TRPV1 has been confirmed.
