Comprehensive Assessment of Authenticating Method of Pathological Staining in Cardiac Fibrosis

VernacularTitle:心肌纤维化病理染色鉴定方法的综合评价
Author: Xian WU ¹ ; Mingjiang LI ; Sha LI
Author Information

1. 430060,武汉大学人民医院心内科、武汉大学心血管病研究所、心血管病湖北省重点实验室
Keywords: Cardiac fibrosis; Collagen; Masson's staining; Picric sirius red staining
From: Journal of Medical Research 2017;46(9):34-37
CountryChina
Language:Chinese
Abstract: Objective To compare hematoxylin and eosin(HE) staining,Masson's staining and picric sirius red(PSR) staining in evaluation of cardiac fibrosis.Methods Twenty adult sprague dawley (SD) rats were given subcutaneous injection of isoprenaline for 5mg/(kg · d) for 3 weeks,which induced cardiac fibrosis model successfully.In order to observing the fibrotic areas and collagen deposition,we prepared paraffin sections by using the heart specimen of rat,and carried out HE staining,Masson staining and PSR staining respectivelly for the special staining.Results All staining methods could clearly show the collagen fibers and fibrosis stage.We could clearly distinguish the type Ⅰ and type Ⅲ collagen fibers through PSR staining with immunoflurescent techniques,but not Masson and HE staining.Conclusion The results demonstrated that PSR staining is better than HE and Masson staining to be used for the evaluation of degree and type of proliferation of collagen fibers.It has important signification for treatment and prevention of cardiac fibrosis.