Polymorphisms of tumor necrosis factor-alpha promotor gene in Kawasaki disease and relation to the risk of coronary artery lesion.
10.3345/kjp.2009.52.4.476
- Author:
Se hwa KIM
1
;
Jang won YUN
;
Young hyuk LEE
;
Eun jung CHEON
Author Information
1. Department of Pediatrics, College of Medicine, Konyang University, Daejeon, Korea. koojook@medimail.co.kr
- Publication Type:Multicenter Study ; Original Article
- Keywords:
Mucocutaneous lymph node syndrome;
Coronary arteries;
TNF-alpha polymorphism
- MeSH:
5' Flanking Region;
Adenine;
Alleles;
Child;
Coronary Vessels;
DNA;
Gene Frequency;
Humans;
Mucocutaneous Lymph Node Syndrome;
Tumor Necrosis Factor-alpha
- From:Korean Journal of Pediatrics
2009;52(4):476-480
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The purpose of this study was to investigate the polymorphisms of the TNF-alpha promotor gene, its susceptibility to Kawasaki disease (KD) and to assess whether the TNF-alpha promotor gene polymorphism was related the risk of coronary artery lesions (CALs). METHODS: From January 2003 to January 2007, 51 children (30 boys and 21 girls) with KD and 48 children forming an age-matched control group were studied. DNA from the peripheral blood of all the children was sampled, and the DNA polymorphisms of the 5' flanking regions of the TNF-alpha promoter gene at position -308 [guanine (G) to adenine (A)] were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Then, the relationship between KD and the TNF-alpha promotor gene polymorphisms was evaluated. RESULTS: The A allele frequency of the -308 site of the TNF-alpha promotor gene was 17.6% (9/51) for children with KD and 6.8% (3/48) for the control group children, but this result was not statistically significant. Twenty-four patients experienced CALs within 60 days after the onset of symptoms. KD children with TNF-alpha -308 A allele had lower frequencies of CALs (12.5% versus 22.2%, P>0.05). CONCLUSIONS: The DNA polymorphism of the -308 site TNF-alpha gene was not associated with susceptibility to KD and a risk of CALs. Multicenter, large-scale randomized controlled trials are needed for further study.
