Distribution and typing of animal-derived Salmonella with MLST and serotype
10.3969/j.issn.1002-2694.2017.09.008
- VernacularTitle:山东省动物源沙门氏菌MLST和血清分型与分布研究
- Author:
Cui ZHAO
1
;
Qing ZHANG
;
yuan Shu GUO
;
lu Lu LI
;
jie Yu LIU
;
ming Yong WANG
;
shan Wei CHANG
;
qing Yu LIU
Author Information
1. 山东省泰安市动物卫生监督所
- Keywords:
Salmonella;
serotype;
MLST;
typing
- From:
Chinese Journal of Zoonoses
2017;33(9):793-799
- CountryChina
- Language:Chinese
-
Abstract:
To compare the discriminatory ability of multilocus sequence typing and serotype of animal-derived Salmonella and find its distribution in Shandong Province,78 chicken-origin,56 duck-origin and 20 swine-origin Salmonella were separated from some regions of Shandong Province.Seven conserve sequences of Salmonella were PCR-amplified for MLST and slide agglutination test for serotyping.Results showed that by serotyping,6 serotypes were identified from chicken-origin Salmonella,including 88.5％0 S.enteritidis,5.1％ S.indiana,2.6％ S.thompson,1.3％ S.typhimurium,1.3％ S.senftenberg,1.3％ S.agama.Two serotypes were identified from duck-origin Salmonella,including 67.9％ S.enteritidis,32.1％ S.ty phimurium.Three serotypes were identified from swine-origin Salmonella,including 65％ S.typhimurium,20％ S.derby,and 15％ S.enteritidis.By MLST typing,seven ST types were identified from chicken-origin Salmonella:ST11,ST19,ST26,ST128,ST14,ST17 and Newl.Three ST types were identified form duck-origin Salmonella:ST11,ST19 and New2.Three ST types were identified from swine-origin Salmonella:ST34,ST40 and ST3007.Overall,the types identified with two methods were closed,so MLST and serotype have similar discriminatory ability.
