Characterization and genomic sequencing of PS2, a lytic phage that infects Serratia marcescens clinical isolates
10.3969/j.issn.1002-2694.2017.09.012
- VernacularTitle:沙雷菌裂解性噬菌体PS2生物学特性及其全基因组测序
- Author:
Gai ZHANG
1
;
Jiang Zhen LI
;
Jing JIN
;
wei Shu WANG
;
jian Song CHEN
;
hui Ya LI
;
hai De HUANG
;
ting Xiao WANG
;
mei Shan WANG
;
quan Zhong WANG
Author Information
1. 河南医学高等专科学校病原生物学与免疫学教研室
- Keywords:
Serratia marcescenes;
phage;
characterization;
genomic sequencing
- From:
Chinese Journal of Zoonoses
2017;33(9):814-820
- CountryChina
- Language:Chinese
-
Abstract:
The present study focused on the characterization and genomic sequence of phage PS2 that infects Serratia marcescens clinical isolates.The morphology of phage PS2 was observed with electron microscope.The one-step growth curve,host range,and stability of PS2 were investigated.In addition,Phage DNA was extracted from the purified phage particles using a MiniBEST Viral RNA/DNA Extraction Kit.DNA sample was analyzed by digesting with restriction enzymes.The phage DNA was used for constructing the sequencing library.The library was sequenced on a MiSeqTM platform.The whole genome sequence was obtained by Velvet (version:1.2.08) assembling.Phage PS2 belongs to the Myoviridae family.The linear,circularly permuted,167 266-bp double-stranded DNA genome of PS2 has high similarities to T4-1ike phages.The phage DNA contains 41.7％ GC and 276 ORFs.PS2 exhibited a 21-minute latent period and 70 PFU per cell at burst size when the pathogenic S.marcescens strain S2 served as a host.Further investigation suggested that PS2 is stable in a wide pH range (pH5 to pH10) and at extreme temperatures (50 ℃ and 60 ℃) after incubation alone at different pHs and different temperatures,respectively.The paper focused on the isolation and identification of a novel lytic S.marcescens phage,the biological characteristics,the whole genome sequencing and the preliminary study of bioinformatics,which laid the foundation for deeply analysis to the phage therapy of multi-drug resistant bacteria and the phage biological information.
