Application of iTRAQ multiplex tandem mass spectrometry in the expression of HGF in breast cancer cell invasion
10.3969/j.issn.1673-4130.2017.19.010
- VernacularTitle:iTRAQ多重标记串联质谱技术在乳腺癌细胞侵袭患者HGF表达中的应用
- Author:
Shuquan HE
1
;
Wenbin KUANG
;
Shaowei SHU
;
Weidong LIU
Author Information
1. 广东省深圳市龙华区中心医院检验科
- Keywords:
breast cancer;
iTRAQ marker;
HGF;
proteomics;
Western blot
- From:
International Journal of Laboratory Medicine
2017;38(19):2683-2684,2687
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the clinical value of iTRAQ multiplex tandem mass spectrometry in the detection of the ex-pression of hepatocyte growth factor (HGF) in patients with invasive breast cancer .Methods A total of 35 patients with breast cancer and 30 healthy subjects were selected from January 2014 to October 2016 in this hospital ,the expression of serum HGF in breast cancer patients with different clinical stages and healthy subjects was analyzed by iTRAQ labeling ,mass spectrometry ,library searching and Scqffold software ,and the differential expression of HGF was verified by Western blot .Results A total of 237 pro-teins were identified in the serum samples of this study ,and 89 proteins with strict quantitative criteria ,17 differentially expressed proteins ,included HGF ,were screened for breast cancer patients and healthy controls .iTRAQ markers showed that the expression level of serum HGF in different clinical stage of breast cancer patients was significantly higher than that in healthy subjects (P<0 .05) .The results of Western blot showed that the relative expression level of serum HGF in breast cancer patients was significant-ly higher than that in healthy subjects(P<0 .05) .Conclusion iTRAQ multiplex tandem mass spectrometry is useful for the detection of breast cancer patients with high expression of HGF ,which is of great significance in guiding the clinical treatment of breast cancer .
