Mechanisms of autophagic eukaryotic cells in Acinetobacter microvilli removal and protein histological study on apoptosis induced by macrophages
10.3969/j.issn.1673-4130.2017.19.023
- VernacularTitle:自噬真核细胞在不动杆菌清除中的机制及诱导巨噬细胞凋亡的蛋白质组织学研究
- Author:
Wei LI
1
;
Lianfeng GUO
;
Zhaowei LIU
;
Xinjie YAO
;
Ping LI
;
Qinglu LI
Author Information
1. 河北省衡水市人民医院检验科
- Keywords:
autophagic eukaryotic cells;
Acinetobacter;
macrophages;
protein histology
- From:
International Journal of Laboratory Medicine
2017;38(19):2720-2722
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the mechanisms of autophagic eukaryotic cells in Acinetobacter microvilli removal and protein histological study on apoptosis induced by macrophages .Methods A model of Acinetobacter baumannii infection was established in 24 female OCR mice .The mice were randomly divided into control group (n= 12) and observation group (n= 12) .The control group was injected with normal saline ,and the observation group was injected with autophagy eukaryotic cells ,the histopathological changes of Acinetobacter and the induction of macrophage apoptosis were observed .Results There was no significant difference in the bacterial counts between the two groups of mice immediately after implantation (P>0 .05) ,the bacterial counts in the 24 and 48 h in the observation group was significantly lower than that in the control group (P<0 .05) .The lung tissue of mice in the ob-servation group injected after autophagy was normal ,the alveolar cavity was open ,no abnormal substances were found ,the alveolar wall was not obviously thickened ,and no inflammatory cell infiltration was found in the wall .The mice in the control group were in-jected with normal saline and lacked the ability to remove Acinetobacter ,resulting in a large number of inflammatory cell infiltra-tion ,vasodilatation ,and congestion in some mice .Conclusion Autophagic eukaryotic cells injected with Acinetobacter baumannii can increase the clearance rate ,induce apoptosis of macrophages and improve the quality of Acinetobacter baumannii .
