Synthesis and evaluation of PDLs22 recombinant protein.
10.5051/jkape.2007.37.1.35
- Author:
Kyoung Yeon LEE
1
;
Yong Seok CHOI
;
You Jin LEE
;
Hyun Sook BAE
;
Heung Joong KIM
;
Kwang Hee CHO
;
Hyun Seon JANG
;
Joo Cheol PARK
Author Information
1. Oral Biology Research Institute, Chosun University and the second stage of BK21, Korea. Jcapark@chosun.ac.kr
- Publication Type:Original Article
- Keywords:
PDLs22;
recombinant protein;
solubility;
expression;
UNCL
- MeSH:
Blotting, Western;
Connective Tissue;
Fibroblasts;
Inclusion Bodies;
Molecular Weight;
Periodontal Ligament;
Periodontium;
Regeneration;
Solubility;
Tooth Root
- From:The Journal of the Korean Academy of Periodontology
2007;37(1):35-44
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Periodontal ligament (PDL) is the connective tissue located between the tooth root and alveolar bone. In a previous study, PDLs22 was isolated as a PDL-specific gene by using subtractive hybridization between cultured PDL fibroblasts and gingival fibroblasts. It was also suggested that PDLs22 plays important roles in the development, differentiation and maintenance of periodontal tissues. However, little is known about functional study of PDLs22 using recombinant protein in PDL fibroblast differentiation and periodontium formation. In this study, in order to produce the PDLs22 recombinat protein, PDLs22-expression vector were constructed and expressed its protein in various host cell and temperature conditions. The results were as follows: 1. PDLs22 protein was not strongly expressed in the induction system using pRSET-PDLs22 construct. 2. When the BL21(DE3) pLysS was used as a expression host, PDLS22 protein was strongly expressed in the induction system using pHCEIIBNd-PDLs22 construct. 3. The PDLs22 protein was recognized at a molecular weight of 28 kDa in western blots. 4. Almost of the expressed PDLs22 protein was not soluble and observed like as inclusion body. 5. The protein solubility was not improved after modification of induction time and temperature during PDLs22 protein production. In this study, the system for the PDLs22 protein production was connstructed. However, the results suggest that further studies will be needed to produce the considerable amount of PDLs22 recombinat protein, which can use for the periodontal regeneration.
