Pneumococcal vaccine promotes the proliferation of CRTH2 (CD4+CD294+ Th2)cells
10.3969/j.issn.1671-9638.2017.10.005
- VernacularTitle:肺炎链球菌疫苗抗原促进CRTH2(CD4+CD294+Th2)细胞增殖的实验研究
- Author:
qin Mei TANG
1
;
qing Fa TIAN
;
heng Ju LI
;
qing Hui LI
;
hui Xiao CHENG
Author Information
1. 深圳市龙岗区人民医院
- Keywords:
pneumococcal vaccine;
dendritic cell;
CRTH2 cell;
cell proliferation
- From:
Chinese Journal of Infection Control
2017;16(10):916-919,930
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the proliferation of CRTH2 (CD4+ CD294+ Th2)cells promoted by pneumococcal vaccine through antigen presentation of dendritic cells (DCs),so as to provide a new approach for amplification and sorting of Th2 cells.Methods CDs induced from peripheral blood mononuclear cells were cocultured with T lym-phocytes after loading pneumococcal vaccine antigen,mixed lymphocyte reaction (MLR)was detected by cell count-ing kit-8(CCK8),DCs and CRTH2 cells were analyzed by flow cytometry.Results Pneumococcal vaccine could promote the maturation of DCs,together with TNF-a,it was adjuvant for maturation of DCs.Pneumococcal vaccine antigen-loaded DCs could increase the rate of subsets of CRTH2 cells on day 5([0.93±0.10]%)compared with day 1([0.70±0.02]%),and absolute number also increased (both P <0.05).Conclusion Amplification of CRTH2 cells can be greatly promoted by pneumococcal vaccine antigen-loaded DCs,which might be one of the effective way to induce amplification of Th2 cells.
