A study on the differentiation of MC3T3-E1 incubated on the layer-built silica/polycaprolactone non-woven fabric produced by electrospinning.
10.5051/jkape.2007.37.1.115
- Author:
Min Kuk AN
1
;
Kyoung Hwa KIM
;
Tae Il KIM
;
Yong Moo LEE
;
Sang Hoon RHEE
;
Young KU
;
In Chul RHYU
;
Chong Pyoung CHUNG
;
Soo Boo HAN
;
Yang Jo SEOL
Author Information
1. Department of Periodontology, Korea. yjseol@snu.ac.kr
- Publication Type:Original Article
- Keywords:
electrospining;
silica;
polycaprolactone;
osteoblast;
proliferation;
differentiation
- MeSH:
Cell Culture Techniques;
Cell Proliferation;
Eagles;
Enzyme-Linked Immunosorbent Assay;
Osteoblasts;
Osteocalcin;
Osteopontin;
Silicon Dioxide;
Transplants
- From:The Journal of the Korean Academy of Periodontology
2007;37(1):115-124
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Silica is known as a promising osteoconductive material, and polycaprolactone is a bioactive and degradable material. The purpose of this study was to monitor the differentiation of MC3T3-E1 cells cultured on the layer-built silica/poly caprolactone non-woven fabric produced by electrospinning. Non-woven fabric (silica, polycaprolactone, PSP, SPS) was made by electrospinning and they were inserted in the 48 well cell culture plate. MC3T3-E1 cells were prepared by subculture. Cells were seeded to each well 1x10(5) concentration per well. Dulbecco's modified eagle medium with 10% FBS and 1% antibiotic-antimycotic solution was used. Confocal laser scanning microscope was taken 4 hours after incubation (95% air, 5% CO2, 37degrees C). Cell proliferation was monitored by spectrophotometer on 1, 7, 14 days, and the morphology of the growing cells was observed by field emission scanning electron microscope. To monitor the differentiation of osteoblasts on the materials, MC3T3-E1 cells were incubated in 48 well culture plate after seeding with the density of 1x10(5) concentration. Then ELISA kit & EIA kit were used on to assess osteocalcin and osteopontin expression respectively. The other conditions were the same as above. MC3T3-E1 cells were proliferated well on all of the materials. There were no statistical differences among them. The osteopontin expression of silica, PSP, SPS was significantly higher than other groups on day 3 (p<0.05), but after that time, there were no statistically signigicant differences. The osteocalcin expression was significantly higher in silica and PSP than other groups on day 14. These findings show that PSP was as good as silica on the effect of osteoblast differentiation. The PSP non-woven fabric may have the possibility as bone graft materials.
