Transcription factor Sp1 in regulation of transcription of VEGF in hypoxia-induced hepatocellular cells
10.7644/j.issn.1674-9960.2017.07.005
- VernacularTitle:Sp1在低氧肝癌细胞血管内皮生长因子转录调控中的作用
- Author:
rong Yu WANG
1
;
yan Yan LI
;
long Ren LU
Author Information
1. 江西省九江市第一人民医院消化科
- Keywords:
hypoxia;
carcinoma;
hepatocellular;
HepG2;
Sp1 transcription factor;
vascular endothelial growth factors ( VEGF);
plicamycin
- From:Military Medical Sciences
2017;41(7):572-575
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of Sp1 in the regulation of transcription of vascular endothelial growth factor ( VEGF ) in hypoxia-induced HepG2 cells.Methods Quantitative real-time polymerase chain reaction and immunoblot assay were used to examine the effect of hypoxia on Sp1 protein and VEGF mRNA expression .Mithramycin A, the selective inhibitor of Sp1 and knock-out Sp1 gene with siRNA were used to examine the effect on VEGF mRNA expression induced by hypoxia in HepG2 cells.Results Hypoxia induced Sp1 protein and VEGF mRNA expression in HepG2 cells.Mithramycin A produced a concentration-dependent decrease of hypoxia-induced VEGF mRNA expression . After inhibition of Sp1 RNAi, VEGF mRNA expression was significantly repressed in HepG 2 cells.Conclusion Hypoxia can increase the expression of Sp1 protein and VEGF mRNA in HepG2 cells induced by hypoxia.The transcription of VEGF is regulated by Sp1 in hypoxia-induced HepG2 cells.
