Development of an attenuated vaccine strain from a korean respiratory type infectious bronchitis virus.
- Author:
Kang Seuk CHOI
1
;
Woo Jin JEON
;
Eun Kyoung LEE
;
Soo Jeong KYE
;
Mi Ja PARK
;
Jun Hun KWON
Author Information
1. Avian Diseases division, Department of Animal and Plant Health Research, Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang 430-757, Korea. kchoi0608@korea.kr
- Publication Type:Original Article
- Keywords:
attenuation;
infectious bronchitis;
live vaccine;
protection
- MeSH:
Chickens;
Eggs;
Humans;
Infectious bronchitis virus;
Korea;
Massachusetts;
Ovum;
Parents;
Point Mutation;
Sprains and Strains;
Vaccination;
Viral Load;
Viruses
- From:Korean Journal of Veterinary Research
2011;51(3):193-201
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
An attenuated vaccine strain AVR1/08 of Korean respiratory type of infectious bronchitis virus (IBV) was developed by 89th passages of IBV D85/06 strain in chicken eggs. The AVR1/08 strain had higher virus titer at least 20 times (10(1.3)) than the parent virus D85/06 by egg inoculation method. The AVR1/08 strain had a single point mutation (S to Y) at position 56 of spike protein of IBV compared to parent virus IBV D85/06 strain. The mutation was observed consistently at viruses after 47th passage in chicken eggs. The AVR1/08 strain showed no virulence even after 6 passages in chickens and all chickens inoculated induced anti-IBV antibody 14 days after vaccination. The AVR1/08 strain had broad protective efficacy against QX type Korean nephropathogenic virus (Q43/06 strain), KM91 type Korean nephropathogenic virus (KM91 strain) and Korean respiratory virus (D85/06 strain). In contrast, Massachusetts (Mass) type attenuated vaccine strain H120 showed protection of 37.5 to 50% against these three viruses. Our results indicate that the AVR1/08 strain has potential as an attenuated vaccine effective in controlling IBVs circulating in Korea.
