Isolation and characterization of fowl adenovirus serotype 4 from chickens with hydropericardium syndrome in Korea.
- Author:
Hong Su PARK
1
;
Il Soo LIM
;
Sang Kyu KIM
;
Toh Kyung KIM
;
Sang Geon YEO
Author Information
1. College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea. sgyeo@knu.ac.kr
- Publication Type:Original Article
- Keywords:
adenovirus properties;
fowl adenovirus;
polypeptides;
RFLP patterns
- MeSH:
Adenoviridae;
Adenoviridae Infections;
Cell Culture Techniques;
Cesium;
Chickens;
Chlorides;
Chloroform;
Electrophoresis;
Embryonic Structures;
Ethanol;
Ether, Ethyl;
Formaldehyde;
Genome;
Hepatocytes;
Hot Temperature;
Hydrogen-Ion Concentration;
Korea;
Liver;
Magnesium Chloride;
Microscopy, Electron, Transmission;
Peptides;
Polymorphism, Restriction Fragment Length;
Sodium;
Trypsin;
Virion;
Viruses
- From:Korean Journal of Veterinary Research
2011;51(3):209-216
- CountryRepublic of Korea
- Language:English
-
Abstract:
Four strains of fowl adenovirus (FAdV) were isolated from 4 flocks of broiler or layer chickens affected by hydropericardium syndrome in Korea. These FAdVs were classified as serotype 4 by restriction fragment length polymorphism patterns of hexon genes and whole genomes. The virus exhibited cytopathic effects consisting of rounding, ballooning and clustering in primary chicken embryo liver cell cultures. In transmission electron microscopy, virus particles in hexagonal shape aggregated exclusively in the nuclei of hepatocytes of the chickens as the typical appearances in adenovirus infections. Buoyant density of the virus in cesium chloride (CsCl) was 1.34 g/mL. The virus was stable to chloroform, ether, 50~70% ethanol, acidic condition at pH 3, 0.25% trypsin (1 : 250), heat at 50degrees C for 30 min, but labile to 100% ethanol, heat at 52~60degrees C for 30 min, 1 M MgCl2 at 50degrees C for 1 h, 1 : 2,000 formalin (37%). All of the physicochemical properties pertained to the characteristics of adenoviruses. Eight viral polypeptides were determined in CsCl-purified virus by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
