Vasorelaxant Effects of the Isoflavones in Red Clover on Isolated Rat Mesenteric Artery in an Endothelium-dependent Manner
- VernacularTitle:红车轴草总异黄酮通过内皮依赖途径对离体大鼠肠系膜动脉舒张作用研究
- Author:
Tao SUN
1
;
Peng YANG
;
Mengmeng YANG
;
Yajuan TANG
;
Yan ZHANG
Author Information
1. 第四军医大学唐都医院药学部 西安710038
- Keywords:
Isoflavones in red clover;
Mesenteric artery;
Artery endothelium;
Nitric oxide
- From:
China Pharmacist
2017;20(10):1710-1712,1717
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the vasorelaxant effect of the isoflavones in red clover on rat mesenteric artery rings. Meth-ods:The rat mesenteric artery rings were placed between two L form metal needles. When stable precontractions induced by vasocon-strictor were got, the different concentrations of isoflavones in red clover and ( or) blocker were added to the baths. The isometric ten-sion of the rat mesenteric artery rings was recorded using a myograph system. Results:The isoflavones in red clover could concentra-tion-dependently relax the rat mesenteric artery rings pre-contracted by K+krebs solution (60 mmol·L-1K+), and compared with the control group (Dmso), the difference was statistically significant (P<0. 01). The isoflavones in red clover-induced relaxation was re-duced by the removal of endothelium, and compared with the complete endothelial group, the difference was statistically significant ( P<0. 01). Pretreatment with Nω-nitro-L-arginine-methylesterhydrochloride (L-NAME,10 -4 mol·L-1) could suppress the relaxing re-sponse to the isoflavones in red clover, and compared with the control group, the difference was statistically significant (P<0. 01). However, indomethacin(10 -5 mol·L-1) did not significantly affect the relaxation induced by the isoflavones in red clover (P >0. 05). Conclusion:The vasorelaxant effect of the isoflavones in red clover is through promoting nitric oxide release from vascular en-dothelium.
