Extraction Optimization of Quercetin and Kaempferol from Lindera Aggregate Leaves by Response Surface Method
- VernacularTitle:响应面法优化乌药叶中槲皮素、山奈酚提取工艺
- Author:
Xingxing YAN
1
,
2
;
浙江中医药大学
;
Xianke YUE
;
Xiaohong CHEN
;
Fangkun ZHANG
;
Liu YANG
Author Information
1. 浙江中医药大学附属第一医院 杭州310018
2. 浙江中医药大学
- Keywords:
Lindera aggregate leaves;
Quercetin;
Kaempferol;
Response surface method;
Extraction
- From:
China Pharmacist
2017;20(10):1731-1736
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To obtain the optimum extraction conditions for quercetin and kaempferol from Lindera aggregate leaves. Methods:On the basis of single factor investigation, Box-Behnken experimental design was used to provide the experimental data for establishing a regression model for the extraction of quercetin and kaempferol. Response surface and contour diagrams with the extrac-tion yield of quercetin and kaempferol as the response values,were plotted for analyzing the pairwise interactive effects of hydrochloric acid concentration, hydrolysis temperature and hydrolysis time. Results:The hydrochloric acid concentration of 4. 1%, the hydrolysis temperature of 83℃ and the hydrolysis time of 45min were the optimum extraction conditions. With the above conditions, the content of quercetin and kaempferol from Lindera aggregate leaves was 6. 97 mg·g-1 and 2. 82 mg·g-1 , respectively. The Lindera aggregate beaves from five different habitals were analyzed,and the total content of quercetin and kaempferide from Taizhou Tiantai were highest. Conclusion:The quercetin and kaempferol extraction from Lindera aggregate leaves is optimized by Box-Behnken response surface method and the process is convenient and feasible.
