Study on the Secondary Metabolites of Endophytic Fungus Alternaria Solani from Aconitum Tsaii
- VernacularTitle:碧江乌头内生真菌Alternaria solani次生代谢产物研究
- Author:
Guiqiong LI
1
;
Chengxin GUO
;
Li HUANG
;
Xiaoyan DUAN
;
Wangyan LIU
;
Dan HE
;
Yong SHEN
Author Information
1. 云南农业大学农学与生物技术学院 昆明650201
- Keywords:
Aconitum tsaii;
Endophytic fungus;
Secondary metabolites
- From:
China Pharmacist
2017;20(10):1760-1764
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the secondary metabolites of endophytic fungus Alternaria solani from Aconitum Tsaii. Methods:The endogenous fungui fermentation product was extracted by methanol followed by recycling methanol to obtain the extract, and the ex-tract was extracted by ethyl acetate to obtain the crude extract. The compounds were isolated and purified by column chromatography and identified based on the spectral analysis ( MS, 1 H-NMR and 13 C-NMR) . Results:Totally 12 compounds were isolated from A. so-lani and characterized as (22E, 24R)-Ergosta-4, 6, 8 (14), 22-tetraen-3-one (1), 1,3-Diolein (2), 5-(3', 3'-Dimethylallyloxy)-3-methoxy-4-methylphthalide (3), pseudomonas aeruginosa H2-sesquiterpene lactone (4), ergosterol peroxide (5), 7-dehydroxyl-zinni-ol (6), ergosterol-7, 22-diene-3β, 5,6-triol (7), 9-octadecenoic acid -2',3'-dihydroxy propyl ester (8), 8-O-Methyltalatisamine (9), Chasmanine (10), yunaconitine (11) and crassicauline A (12). Conclusion:Totally 12 compounds are isolated from the endo-phytic fungus Alternaria solani, and four of them are isolated for the first time.
