Comparison of Protein Content Determination Respectively by Folin-Ciocalteu Method and Coomassie Bril-liant Blue Binding Method for Mannatide Oral Solution
- VernacularTitle:福林酚法与考马斯亮蓝法测定甘露聚糖肽口服溶液中蛋白质含量的比较
- Author:
Jianhua HE
1
;
Lin LU
;
Chunjun SHAO
;
Guilian XU
Author Information
1. 大连市药品检验所 辽宁大连116021
- Keywords:
Folin-Ciocalteu method;
Coomassie brilliant blue binding method;
Mannatide;
Protein
- From:
China Pharmacist
2017;20(10):1861-1863
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the protein content determination method respectively by Folin-Ciocalteu method and Coomass-ie brilliant blue binding method for mannatide oral solution and compare the results. Methods:The limit of detection, linearity, accu-racy, repeatability, recovery and content of Folin-Ciocalteu method and Coomassie brilliant blue binding method were investigated. Re-sults:As for Folin-Ciocalteu method, the limit of detection was 0. 2 μg, the range of linearity was 0-112. 0 μg(r=0. 9990), the av-erage recovery was 105. 2%(RSD=1. 9%), the RSD of accuracy was less than 1%, and the content result of three batches of samples was 47. 45, 58. 34 and 40. 99 μg·ml-1, respectively. As for Coomassie brilliant blue binding method, the limit of detection was 0. 8 μg, the range of linearity was 0-99. 6 μg(r=0. 9980), the average recovery was 102. 0%(RSD=2. 7%), the RSD of accuracy was less than 1%, and the protein content of samples was not detected out by the method. Conclusion:Folin-Ciocalteu method can be used to determine the protein content in mannatide oral solution reliably and efficiently.
