Determination of Five Index Components in Yinzhihuang Oral Liquid by Quantitative Analysis of Multi-components by Single Marker
- VernacularTitle:一测多评法同时测定茵栀黄口服液中5种指标性成分的含量
- Author:
Weiwen ZHANG
1
Author Information
1. 南通卫生高等职业技术学校 江苏南通226000
- Keywords:
Quantitative analysis of multi-components by single marker;
Yinzhihuang oral liquid;
Chlorogenic acid;
Geniposide;
Hyperin;
Baicalin;
Galuteolin
- From:
China Pharmacist
2017;20(10):1764-1768
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop a method of quantitative analysis of multi-components by single marker ( QAMS) for five index components in Yinzhihuang oral liquid. Methods:Using baicalin as the reference,an HPLC method with a Venusil MP C18 (2) column (250 mm × 4. 6 mm,5 μm) was applied,the mobile phase was acetonitrile-0. 1% phosphoric acid with gradient elution,the flow rate was 1. 0 ml·min-1 ,the detection wavelength was 327 nm, the column temperature was 35℃ and the injection volume was 10μl. The relative correction factors among the five index components were detected by QAMS. The five index contents were determined respec-tively by an external standard method and QAMS, and the results obtained from the two different methods were compared to verify the practicability and stability of QAMS. Results: The established QAMS method was used to determine the five index components in 6 batches of Yinzhihuang oral liquid. There were no significant differences in the calculated values and the determined ones (P>0. 05). Conclusion:The QAMS method is simple,effective and accurate in determining the contents of five index components in Yinzhihuang oral liquid,which can be used for the quality control of Yinzhihuang oral liquid and provide reference for the further studies.
