Simultaneous Determination of Five Components in Guilong Kechuanning Tablets by UPLC with Wave-length Switching
- VernacularTitle:UPLC波长切换法同时测定桂龙咳喘宁片中5种成分的含量
- Author:
Chengyan ZHU
1
;
Chunling ZHOU
;
Huibo YANG
Author Information
1. 西峡县食品药品检验所 河南南阳474500
- Keywords:
Guilong Kechuanning tablets;
UPLC;
Wavelength switching;
Paeoniflorin;
Berberine hydrochloride;
Alkaloid;
Cin-namic acid;
Cinnamaldehyde
- From:
China Pharmacist
2017;20(10):1871-1873
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop a UPLC method for the simultaneous determination of 5 components in Guilong Kechuanning tablets including paeoniflorin, berberine hydrochloride, alkaloid, cinnamic acid and cinnamaldehyde. Methods:An ACQUITY UPLC BEH C18(2.1 mm ×100 mm,1.7 μm)chromatographic column was used;the mobile phase was acetonitrile(A)–0.1% formic acid solution (B) with gradient elution (0-10 min, 85% A;10-13 min, 10% A;13-15 min, 85% A) at a flow rate of 0. 4 ml·min-1, the detection wavelengths were:0-1. 8 min, 230 nm;1. 8-6. 0 min, 345 nm;6. 0-9. 0 min, 285 nm;9. 0-12. 0 min, 345 nm, and the column temperature was 30℃. Results:The linear range of paeoniflorin, berberine hydrochloride, alkaloid, cinnamic acid and cinna-maldehyde was 0. 060-1. 202 μg(r=0. 9999),0. 100-2. 010 μg(r=0. 9999),0. 040-0. 794 μg(r=0. 9994),0. 015-0. 302 μg(r=0.9999) and 0.042-0.850 μg(r =0.9999), the average recovery (n = 6) was 99.63%,99.26%,100.17%,98.80% and 100. 26%, and the RSDs were 0. 39%,0. 97%,0. 73%,1. 00% and 0. 71%, respectively. Conclusion:The method is simple, accu-rate and reproducible. It can be used for the quality control of Guilong Kechuanning tablets.
