Improvement of the Quality Standard for Jinhuanglian Oral Liquid
- VernacularTitle:金黄连口服液质量标准的提高
- Author:
Long LI
1
,
2
;
第四军医大学西京医院药剂科
;
Shanbo MA
;
Jin WANG
;
Juan BAI
;
Ying CHEN
;
Xiaopeng SHI
;
Zhifu YANG
;
Aidong WEN
;
Jinyi CAO
Author Information
1. 陕西中医药大学药学院 陕西咸阳712083
2. 第四军医大学西京医院药剂科
- Keywords:
Jinhuanglian oral liquid;
Quality standard;
HPLC;
TLC
- From:
China Pharmacist
2017;20(10):1876-1878
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To improve the quality standard for Jinhuanglian oral liquid. Methods:The three herbs in the preparation, Honeysuckle, Scutellaria Baicalensis Georgir and St John's Wort, were identified by TLC qualitatively. The content of chlorogenic acid in Lonicerae Japonicae Flos was determined by HPLC. The chromatographic separation was carried out on a Promosil C18 (250 mm × 4. 6 mm,5 μm) column at 25℃. The mobile phase consisted of acetonitrile-0. 4% phosphoric acid (10 :90) with gradient elution at a flow rate of 1. 0 ml·min -1 , and the injection volume was 10μl. The detection wavelength was 327 nm. Results:The spots in TLC were clear without any interference. The linear range of chlorogenic acid was 5.325-170.400 μg·ml-1(r=0.9998). The average recovery was 97. 95% and the RSD was 2. 04%(n=6). Conclusion:The method is reliable and easy to operate,which can be used as the quality control method for Jinhuanglian oral liquid.
