Simultaneous Quantitative Determination of Five Saponins in Notoginseng Total Saponins by HPLC with a Monolithic Column
- VernacularTitle:整体柱HPLC法测定三七总皂苷中的5种皂苷成分
- Author:
Yuanjie ZHANG
1
;
Lan ZHOU
;
Guofang LAI
Author Information
1. 云南省品药品监督检验研究院 昆明650011
- Keywords:
Notoginseng total saponins;
HPLC;
Monolithic column
- From:
China Pharmacist
2017;20(10):1879-1881
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a rapid HPLC method for the quantitative determination of 5 saponins ( notoginsenoside R1 , ginsenoside Rg1 ,ginsenoside Re,ginsenoside Rb1 and ginsenoside Rd) in Notoginseng total saponins using a monolithic column. Meth-ods:The analysis was performed on a Merck Chromolith Performance RP-18e column (100 mm × 4. 6 mm,2μm) with gradient elution of acetonitrile and water. The detection wavelength was set at 203 nm. Results:Satisfactory separation of all analytes was obtained in 20 min. All calibration curves showed good linearity within the testing ranges (r≥0.9998). The average recoveries were between 98. 6% and 100. 4%. The RSDs were less than 2. 1% (n=6). Conclusion:The method is efficient and accurate for the quality con-trol of Notoginseng total saponins.
