Studies on anti-oral cancer activities of medicinal plant extracts.
- Author:
Young Hoon LEE
1
;
Yeo Gab KIM
;
Jung Hee KIM
Author Information
1. Department of OMFS, College of Dentistry, Kyung-Hee University.
- Publication Type:Original Article
- Keywords:
Anti-oral cancer activity;
medicinal plant extracts;
IC50
- MeSH:
Bone Marrow;
Caesalpinia;
Cell Survival;
Cisplatin;
Coptis;
Curcuma;
Humans;
Inhibitory Concentration 50;
KB Cells;
Lepidium;
Lonicera;
Mass Screening;
Methanol;
Mouth Neoplasms;
Plants, Medicinal*;
Solanum nigrum
- From:Journal of the Korean Association of Oral and Maxillofacial Surgeons
2000;26(1):53-58
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Treatment of oral cancers with chemotherapeutic agents are evaluated as an effective method for remission to reduce cancer proliferation nowadays. But, minimization of side-effects such as bone marrow suppression, gastrointestinal toxicity and renal damage is another problem to be solved. Thus, a possible approach to develop a clinically applicable chemotherapeutic agents is to screen anticancer activity among traditional medicinal plants which have been used for thousands of years with very low side-effects in orient. In this study we focused on screening anti-oral cancer activities among 14 traditional medicinal plant extracts that revealed anticancer activities on other solid tumors. The results were as follow: 1. Methanol extract of Lepidium apetalum showed the highest anti-oral cancer activity against A253 cells. At concentration of 4 microgram/ml, the cell viability was 48% under our experimental condition. IC50 value obtained was 4 microgram/ml. 2. Methanol extract of Coptis japonica and Solanum nigrum were effective on KB cells. Cell viability observed were 62% and 67% at concentration of 4 microgram/ml, and IC50 values were 12 microgram/ml and 10 microgram/ml respectively. 3. When the methanol extract of Lonicera caerule was combined with 2 microgram/ml of cisplatin, the anticancer activity was synergistically increased. One hundred microgram/ml of Lonicera caerule showed 92% (alone) or 59% (combined with cisplatin) cell viabilities. IC50 value of Lonicera caerule extract against KB cells was reduced from 301 microgram/ml to 126 microgram/ml when combined with 2 microgram/ml of cisplatin. 4. Medicinal plant extracts effective on both A253 and KB cells were Coptis japonica, Lepidium apetalum, Solanum nigrum, Caesalpiniae Lignum, Curcuma aromatica.
