In situ hybridization Study on the Age-related Changes of Ghrelin mRNA Expression in Rat Stomach.
- Author:
Mi Haeng CHO
1
;
Sang Hyuk LEE
;
Nam Hee KIM
;
Hyun Joon SOHN
;
Jeonghyun PARK
;
Seung Jun HWANG
;
Min Seon KIM
;
Kyeong Han PARK
Author Information
1. Department of Anatomy, Seoul National University College of Medicine. insitu@snu.ac.kr
- Publication Type:Original Article
- Keywords:
Ghrelin;
Stomach;
Rat;
mRNA;
In situ hybridization
- MeSH:
Amino Acids;
Animals;
Eating;
Endocrine Cells;
Gastric Acid;
Gastric Mucosa;
Ghrelin*;
Growth Hormone;
In Situ Hybridization*;
Intestines;
Neck;
Rats*;
RNA, Messenger*;
Stomach*
- From:Korean Journal of Anatomy
2003;36(3):167-173
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Ghrelin is a recently identified member of growth hormone secretagogue, which is synthesized as peptide of 28 amino acids in stomach, pituitary and intestines. Ghrelin controls food intake, and induces gastric acid secretion. It has been reported that synthesis and secretion of ghrelin changes according to age of animals. In this study, in situ hybridization technique with digoxigenin-labeled oligonucleotide probe is applied to elucidate the developmental change of ghrelin mRNA expression pattern in rat stomach. Ghrelin mRNA positive cells first appeared in stomach of 9 day rats, mainly in base of gastric glands. It showed the highest density and spread to neck area of the gastric gland in 6 week rats. Number of positive cells decreased significantly in 18 months. Most of ghrelin mRNA positive cells were found as closed-type endocrine cells in fundus area. These results suggest that ghrelin expression may be involved in development and ageing process.
