Minocycline Protects Vestibular Hair Cells from Neomycin-Induced Ototoxicity by Inhibiting Reactive Oxygen Species Production and Caspase-3 Activity.
- Author:
Young Saeng KIM
1
;
Jin Chul AHN
;
In Yeong BAEK
;
Chung Ku RHEE
;
Jae Yun JUNG
Author Information
1. Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Dankook University, Cheonan, Korea. jjking@dankook.ac.kr
- Publication Type:Original Article
- Keywords:
Minocycline;
Caspase-3;
Reactive oxygen species;
Utricle
- MeSH:
Aminoglycosides;
Animals;
Caspase 3*;
Cell Count;
Hair;
Hair Cells, Vestibular*;
Hearing Loss;
Minocycline*;
Neomycin;
Rats;
Reactive Oxygen Species*;
Saccule and Utricle;
Tetracycline
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2006;49(12):1159-1164
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: Aminoglycosides, a commonly used antibiotic agent, destroys the sensory hair cells in the cochlear and vestibular system leading to irreversible hearing loss and balance problem. Minocycline, a second-generation tetracycline antibiotic, has been known to possess anti-apoptotic properties in addition to its antimicrobial action. We hypothesized that minocycline would attenuate aminoglycosides induced vestibulotoxicity in rat utricles. SUBJECTS AND METHOD: Utricular maculae prepared from postnatal day 3-4 rats were treated with neomycin alone or in combination with minocycline. For hair cell count, utricles were stained with phalloidin-FITC. Reactive oxygen species (ROS) was assessed using the fluorescent probe, hydrofluorescent diacetate acetyl ester (H2DCFDA). Caspase-3 activity was also examined using the fluorescent caspase-3 substrate. RESULTS: Neomycin induced dose-dependent loss of utricular hair cells. Minocycline reduced ROS production and caspase-3 activation in neomycin treated utricular hair cells. CONCLUSION: Minocycline has protective effect in neomycin induced ototoxicity in rat utricle by inhibiting ROS production and caspase-3 activity.
