Collagen Synthesis in an in Vivo Rat Model of Ventilator-induced Lung Injury.
- Author:
Won Il CHOI
1
Author Information
1. Department of Internal Medicine, Keimyung University School of Medicine, Daegu, Korea. wichoi@dsmc.or.kr
- Publication Type:Original Article
- Keywords:
Collagen;
Lung compliance;
Ventilator-induced lung injury
- MeSH:
Acute Lung Injury;
Animals;
Collagen Type I;
Collagen*;
Compliance;
Immunohistochemistry;
Lung;
Lung Compliance;
Models, Animal*;
Rats*;
Respiration, Artificial;
Tidal Volume;
Ventilation;
Ventilator-Induced Lung Injury*
- From:The Korean Journal of Critical Care Medicine
2006;21(2):109-115
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Experimentally, maintaining high pressure or high volume ventilation in animal models produces an acute lung injury, however, there was little information on remodeling. We investigated the collagen synthesis in a rat model of ventilator-induced lung injury. METHODS: Rats were ventilated with room air at 85 breaths/minute for 2 hours either tidal volume 7 ml/kg or 20 ml/kg (V(T)7 or V(T)20, respectively). After 2 hours of ventilation, rats were placed in the chamber for 24 hours. Lung collagen was evaluated by immunohistochemistry (n=5) and collagen was quantitated by collagen assay (n=5). Static compliance (Csta) of the whole lung as obtained from the pressure volume curves. RESULTS: Type I collagen was an increase in expression in the interstitium with large V(T) (20 ml/ kg) ventilation after 2 hours of mechanical ventilation (MV), and further increased expression after 24 hours of recovery period. Static lung compliance was significantly (p<0.05) decreased in the V(T)20 compared with V(T)7 (0.221+/-0.05 vs 0.305+/-0.06 ml/cm H2O) after 2 hours of MV. There was a further decrease in lung compliance after 24 hours of recovery period (0.144+/-0.07 vs 0.221+/-0.05, p<0.05) in the V(T)20. CONCLUSIONS: Large tidal volume ventilation causes an increase in type 1 collagen expression with reduction of lung compliance.
