Screening of Low Molecular Metabolite, FS390 as an Inhibitor of Neurotransmitter Release from PC12 Cells.
- Author:
Yeun Tai CHUNG
1
;
Hee Jung KIM
;
Yun Sik LEE
Author Information
1. Department of Biology, College of Natural Science, Chonbuk National University, Jeonju 561-756, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
PC12 cells;
Neurotransmitter release;
Exocytosis;
Streptomyces spp.;
Inhibitor
- MeSH:
Adenosine Triphosphate;
Animals;
Exocytosis;
Ionomycin;
Mass Screening*;
Neurons;
Neurotransmitter Agents*;
Norepinephrine;
PC12 Cells*;
Rats;
Scintillation Counting;
Streptomyces
- From:Korean Journal of Anatomy
2006;39(2):91-102
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
We established an in vitro experimental system using the following procedure. We first introduced tritium-labeled norepinephrine ([3H]-NE) into PC12 cells. The [3H]-NE incorporated-PC12 cells were stimulated by a high concentration (60 mM) of K+ buffer during 12 minutes. Then, we collected 100 microliter supernatant and counted the amount of [3H]-NE release from PC12 cells with a scintillation counter. After screening fungal, Streptomyces spp. or bacterial product using this experimental sytem, we obtained FS390 from Streptomyces spp. which inhibited [3H]-NE release from PC12 cells. FS390 also inhibits the release of ATP as a neurotransmitter of PC12 cells and rat cortical neurons. The inhibitory effect was seen even when the PC12 cells were treated with low K+ buffer containing ionomycin (1 micrometer) as an ionopore. This result suggests that the inhibitory action of FS390 on neurotransmitter release appeared after the influx of Ca2+.
