Inhibition of p65 Nuclear Translocation by KIOM-79.
- Author:
Jin Sook KIM
1
;
Dae Sik JANG
;
Young Jin JEON
;
Ho Jin YOU
;
Kyeong Han PARK
;
Jin MING
;
Hyung Yoon MOON
;
Sang Pil YOON
;
In Youb CHANG
Author Information
1. Department of Herbal Pharmaceutical Development, Korea Institute of Oriental Medicine, Korea.
- Publication Type:Original Article
- Keywords:
KIOM-79;
Macrophages;
iNOS;
NF-kappaB/Rel
- MeSH:
DNA;
Euphorbia;
Gene Expression;
Glycyrrhiza uralensis;
Macrophages;
Magnolia;
Negotiating;
Nitric Oxide;
Pueraria
- From:Korean Journal of Anatomy
2006;39(2):103-110
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
We demonstrate that KIOM-79, combined extracts isolated from Magnolia officinalis, Pueraria lobata, Glycyrrhiza uralensis, and Euphorbia pekinensis, inhibits LPS-induced expression of iNOS gene in RAW 264.7 cells. Treatment of RAW 264.7 cells with KIOM-79 inhibited LPS-stimulated nitric oxide production in a doserelated manner. Immunohisto-chemical staining of iNOS and RT-PCR analysis showed that the decrease of NO was due to the inhibition of iNOS gene expression. Immunostaining of p65 and EMSA showed that KIOM-79 inhibited NF-kappa/Rel nuclear translocation and DNA binding, respectively. Collectively, this series of experiments indicates that KIOM inhibits iNOS gene expression by blocking NF-kappa/Rel. Due to the critical role that NO release plays in mediating inflammatory responses, the inhibitory effects of KIOM-79 on iNOS suggest that KIOM-79 may represent a useful anti-inflammatory agent.
