Isolation of cholesterol-lowering lactic acid bacteria from human intestine for probiotic use.
- Author:
Hyeong Jun LIM
1
;
So Young KIM
;
Wan Kyu LEE
Author Information
1. College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, Korea. wklee@cbu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Lactic acid bacteria (LAB);
probiotics;
cholesterol;
bile salt hydrolase (BSH)
- MeSH:
Amidohydrolases/metabolism;
Bifidobacterium/*isolation&purification/physiology;
Cholesterol/*metabolism;
Feces/microbiology;
Female;
Humans;
Intestines/*microbiology;
Lactobacillus/*isolation&purification/physiology;
Male;
Probiotics/*therapeutic use;
Streptococcus/*isolation&purification/physiology;
Taurodeoxycholic Acid
- From:Journal of Veterinary Science
2004;5(4):391-395
- CountryRepublic of Korea
- Language:English
-
Abstract:
Cholesterol-lowering effect of lactic acid bacteria (LAB: Streptococcus, Lactobacillus and Bifidobacterium) is well-known. Thus, we investigated LAB isolated from human intestine on the cholesterol-lowering effect in vitro. Seven Streptococcus (61.1%), 11 Lactobacillus (71.8%) and 7 Bifidobacterium (27.9%) were isolated as acid (pH 2.5 and 3.0) and bile (0.3% oxgall) tolerant strains. Streptococcus HJS-1, Lactobacillus HJL-37 and Bifidobacterium HJB-4 were finally selected as probiotic strains to use through the bile salt hydrolase (BSH) activity assay by using MRS media added taurodeoxycholic acid (TDCA) and the cholesterol-lowering test by using soluble cholesterol containing MRS broth. These studies suggested that the isolated LAB had an excellent hypocholesterolemic effect.
