Role of Human Papillomavirus Infection in Laryngeal Cancer.
- Author:
Hyung Kyung JUN
1
;
Soon Yuhl NAM
;
Hyuk KIM
;
Sang Yoon KIM
;
Joo Ryung HUH
Author Information
1. Department of Otolaryngology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea. synam@www.amc.seoul.kr
- Publication Type:Original Article
- Keywords:
Human papillomavirus;
Laryngeal cancer;
PCR;
Dot blot hybridization
- MeSH:
Alcohol Drinking;
Carcinogenesis;
Carcinoma, Squamous Cell;
Consensus;
DNA;
Follow-Up Studies;
Genome;
Genome, Viral;
Humans*;
Incidence;
Laryngeal Neoplasms*;
Papillomavirus Infections*;
Polymerase Chain Reaction;
Recurrence;
Risk Factors;
Smoking
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
1998;41(4):512-517
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: Cigarette smoking and alcohol consumption are well known risk factors of laryngeal squamous cell carcinoma. Recently, attention has been focused on other risk factors such as human papillomavirus (HPV). The presence of HPV genome in the tumor has been reported to correlate with poor patient outcome. The integration of viral genome into the host DNA is believed to be helped by alcohol and cigarette smoking. This study attempted to evaluate the incidence of HPV in laryngeal cancer with regard to its stage and recurrence. MATERIALS AND METHODS: The authors examined the incidence of HPV in 61 cases of laryngeal cancers (34 supraglottic cancers, 26 glottic cancers, 1 subglottic cancer). The histologic type of tumor in all cases was squamous cell carcinoma. There were 8 patients in the stage 1, 26 patients in the stage 2, 14 patients in the stage 3 and stage 4. Recurrence of the disease during follow-up periods occurred in 12 patients (19.6%). Polymerase chain reaction (PCR) was performed using the consensus primers for HPV on the formalin-fixed, paraffin-embedded archival tissue. For cases with amplified products corresponding to the HPV genome, a dot blot hybridization was performed for typing the different HPV types. RESULTS: The HPV genome was identified in 5 cases (8%) of laryngeal squamous cell carcinomas by using PCR. In recurrent laryngeal cancer group, detection rate of HPV DNA was significantly greater than in the non-recurrent group (p<0.05). The dot blot hybridization showed only one case to be the HPV type 31. The other cases (4 cases) could not be typed using the dot blot hybridization. CONCLUSION: We can only say that the possible role of HPV in the carcinogenesis of larynx cancer is not yet resolved.
