Effects of p27 Overexpression on Head and Neck Squamous Cell Carcinoma Cell Lines.
- Author:
Soo Yeun PARK
1
;
Dong Wook LEE
Author Information
1. Department of Anatomy, College of Medicine, Ewha Womans University, Seoul 158-710, Korea. psy03@ewha.ac.kr
- Publication Type:Original Article
- Keywords:
p27;
Head and neck squamous cell carcinoma;
Cell cycle;
Apoptosis
- MeSH:
Apoptosis;
Carcinoma, Squamous Cell*;
Cell Cycle;
Cell Cycle Checkpoints;
Cell Line*;
Cell Proliferation;
Cyclins;
Down-Regulation;
Head*;
Humans;
Neck*;
Phosphotransferases;
Prognosis
- From:Korean Journal of Anatomy
2005;38(3):227-234
- CountryRepublic of Korea
- Language:English
-
Abstract:
p27 is a cyclin dependent kinase inhibitor and plays an important role in negative regulation of the cell cycle. It has been revealed that down-regulation of p27 is frequently found in various cancers and is well associated with their malignancies including poor prognosis. In the present work, we constructed including inhibition of cell proliferation, arrest of cell cycle, and induction of apoptosis in head and neck squamous cell carcinoma (HNSCC)cells. On transduction of the HNSCC cell lines with ad-p27, overexpression of p27 was confirmed in all cell lines tested. In addition, overexpression of p27 resulted in significant inhibition of cancer cell proliferation with increased apoptosis and cell cycle arrest in HNSCC cell lines. From these results, it was suggested that overexpression of p27 could show anti-tumor effects in HNSCC cell lines and it is possible that an increase of p27 protein by introducing the p27 gene into human cancer cells may be helpful for the treatment or prevention of human cancers, especially HNSCC.
