c-kit and Ligand Expression in the Mouse Hippocampus after Kainate-induced Seizure.
- Author:
Hye Nam HONG
;
Jong Hwan LEE
;
Tae Jin JUNG
;
Dong Ho KIM
- Publication Type:Original Article
- Keywords:
c-kit;
KL ligand;
Stem Cell Factor[SCF];
Bcl-2;
Apoptosis;
Kainate-induced seizure
- MeSH:
Animals;
Apoptosis;
Brain;
Cell Survival;
Hippocampus*;
In Situ Hybridization;
Kainic Acid;
Mice*;
Neurons;
Proto-Oncogene Proteins c-kit;
RNA, Messenger;
Seizures*;
Translations
- From:Korean Journal of Anatomy
1997;30(1):87-98
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
In the present study, we have etamined the role of c-kit and KL ligand in the mouse brain after kainate-induced seizure. To investigate whether c-kit receptor and KL ligand might involved in kainate-induced apoptosis, the expression patterns of c-kit and KL mRNA and localization of immunoreactivity for c-Kit, SCF and Bcl-2 protein were examined by in situ hybridization technique and immunohistochemical method, respectively, in the mouse hippocampus after kainate treatment. This report is the first demonstration for the role of c-kit receptor and KL ligand in the kainate-induced apoptosis. Our conclusion is based on : 1] c-kit and KL mRNA expressions were increased in CA3 region of the hippocampus in 1h after kainate treatment, 2] immunoreactivities for c-Kit protein and SCF were detected higher level in the CA1 and CA3 sectors in 24h after kainate treatment, 3] expression level for Bcl-2 protein was increased in the CA3 region of the hippocampus 24h after kainate treatment. These results suggest that bcl-2 could promote cell survival of injured neurons in CA3 after kainate-induced seizure. And increased translations of c-kit receptor and KL ligand after kainate injection in this area susgest that c-kit receptor and KL ligand could have a role in the kainate-induced apoptosis.
