Limb Allotransplantation In Rat Immunosuppressed with FK-506.
10.4055/jkoa.2002.37.5.670
- Author:
Young Ho KWON
1
;
Gu Hee JUNG
;
Jae Do KIM
Author Information
1. Department of Orthopaedic Surgery, College of Medicine, Kosin University, Busan, Korea. yhkwon@ns.kosinmed.or.kr
- Publication Type:Original Article
- Keywords:
Limb allotransplantation;
FK-506
- MeSH:
Allografts;
Animals;
Extremities*;
Graft Rejection;
Humans;
Norway;
Rats*;
Survival Rate;
Tacrolimus*;
Tissue Donors
- From:The Journal of the Korean Orthopaedic Association
2002;37(5):670-677
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: This study investigates the effectiveness of FK-506, a new immunosuppressive agent for in preventing the rejection of limb allo-transplants in rats. MATERIALS AND METHODS: Inbred strain Lewis (RT1l) were used as donors and Brown Norway (RT1n) as recipients for limb allotransplan-tation. The experimental animals were divided into four groups; group I: untreated allografts, group II: allografts receiving FK-506 1mg/kg/day for 5-12 days (n=6), group III: 2 mg/kg/day for 15 days (n=6), group IV: single dose of 10 mg/kg (n=3), immediately. Rejection signs, mean survival time of limbs and histologic analysis (by the muscle grading system of Buttemeyer et al.) were evaluated. RESULTS: The mean survival time in group I was 3.5 +/-1.04 days, in group II 7.17 +/-3.43 days, in group III 25.5 +/-12.01 days and in group IV 16.33 +/-8.08 days. FK-506 prolonged the survival time of limbs significantly in a dose-dependent manner. Histologic examination revealed that group II was grade II (n=2) and grade III (n=1); group III was grade I (n=2); and group IV was grade I (n=1) and grade II (n=1). CONCLUSION: These results demonstrate the unique effects of FK-506 upon preventing graft rejection and prolonging limb survival allo-grafts in rats.
