The Incidence Rate and Severity of Orthotopic Lung Cancer in an Animal Model Depends on the Number of A549 Cells and Transplantation Period.
10.5625/lar.2010.26.4.369
- Author:
Jinsoo LEE
1
;
Young Ah HAN
;
Hyo Seon YANG
;
Jeong Ah SONG
;
Young Su YANG
;
Soonjin KWON
;
Min Sung KANG
;
Kyuhong LEE
;
Jeong Doo HEO
;
Kyu Hyuk CHO
;
Chang Woo SONG
Author Information
1. Inhalation Toxicology Center, Korea Institute of Toxicology Jeongeup Campus, Jeongeup, Korea. cwsong@kitox.re.kr
- Publication Type:Original Article
- Keywords:
Lung cancer animal model;
orthotopic;
A549
- MeSH:
Adenocarcinoma;
Animals;
Carcinoma, Non-Small-Cell Lung;
Cell Count;
Cell Line;
Humans;
Incidence;
Lung;
Lung Neoplasms;
Mice;
Mice, Nude;
Models, Animal;
Transplants
- From:Laboratory Animal Research
2010;26(4):369-375
- CountryRepublic of Korea
- Language:English
-
Abstract:
The incidence rate of lung cancer is continually increasing, and lung cancer is the leading cause of cancer-related death worldwide. Nevertheless, few therapeutic methods are available for lung cancer. Therefore, establishing appropriate lung cancer animal models is important to investigate mechanisms and to evaluate new drugs for lung cancer. In the present study, we transplanted non-small cell lung cancer A549 human adenocarcinoma cells (2x10(4), 2.0x10(5), and 2.0x10(6) cells) into the right lobe of BALB/c nude mice via the intercostal space to develop an orthotopic lung cancer animal model that is minimally invasive and similar to human lung cancer. We then investigated the incidence rate and severity of lung cancer according to the A549 cell number (2x10(4), 2.0x10(5), and 2.0x10(6) cells) and transplantation periods (4~23 days). Lung cancer development was confirmed with gross examination, which was supported by histopathological examination. These results indicate that the incidence rate and severity of lung cancer was increased depending on the number of transplanted cells and transplantation period which the cell number and duration are increasing risk of lung cancer. Thus, this study can provide appropriate reference data to develop an orthotopic lung cancer animal model using the non-small cell lung cancer A549 cell line for researching mechanisms and evaluating candidate drugs, including various approaches for treating lung cancer.
