Effect of6-Gingerol on Inhibition of Cell Proliferation in MDA-MB-231 Human Breast Cancer Cells.
- Author:
Eun Young SEO
1
;
Hyun Sook LEE
;
Woo Kyung KIM
Author Information
1. Department of Food Science and Nutrition, Dankook University, Seoul, Korea. wkkim@dankook.ac.kr
- Publication Type:Original Article
- Keywords:
[6]-gingerol;
breast cancer cell;
proliferation;
EGFR;
Akt
- MeSH:
Breast Neoplasms*;
Breast*;
Cell Line;
Cell Proliferation*;
Colic;
Common Cold;
Condiments;
Ginger;
Herbal Medicine;
Humans*;
Rheumatic Diseases;
Rhizome;
RNA, Messenger;
Spices
- From:The Korean Journal of Nutrition
2005;38(8):656-662
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Ginger (Zingiber officinale Roscoe, Zingiberaceae) is one of the most frequently and heavily consumed dietary condiments throughout the world. Besides its extensive use as a spice, the rhizome of ginger has also been used in traditional oriental herbal medicine for the management of symptoms such as common cold, digestive disorders, rheumatism, neurologia, colic, and motion-sickness. The oleoresin from rhizomes of ginger contains[6]-gingerol (1-[4'-hydroxy-3'-methoxyphenyl]-5-hydroxy-3-decanone) and its homologs as pungent ingredients that have been found to possess many interesting pharmacological and physiological activities, such as anti-inflammatory, analgesic, antipyretic, antiheatotoxic, and cardiotonic effects. However, the effect of[6]-gingerol on cell proliferation in breast cancer cell are not currently well known. Therefore, in this study, we examined effect of[6]-gingerol on protein and mRNA expression associated with cell proliferation in MDA-MB-231 human breast cancer cell lines. We cultured MDA-MB-231 cells in presence of 0, 2.5, 5 and 10 micrometer of[6]-gingerol.[6]-Gingerol inhibited breast cancer cell growth in a dose-depenent manner as determined by MTT assay. ErbB2 and ErbB3 protein and mRNA expression were decreased dose-dependently in cells treated with[6]-gingerol (p < 0.05). In addition, phosphorylated Akt levels and total Akt levels were markedly decreased in cells treated with 2.5 micrometer[6]-gingerol (p < 0.05). In conclusion, we have shown that[6]- gingerol inhibits cell proliferation through ErbB2 and ErbB3, reduction in MDA-MB-231 human breast cancer cell lines.
