The usefulness of multiple displacement amplification in the molecular studies in Hansen's disease.
- Author:
Jong Pill KIM
1
Author Information
1. Institute for Leprosy Research, Korean Hansen Welfare Association, Korea. dr_jpkim@hotmail.com
- Publication Type:Original Article
- Keywords:
MDA (multiple displacement amplification);
Hansen's disease
- MeSH:
Displacement (Psychology);
DNA;
Genome;
Leprosy;
Mycobacterium leprae;
Pathology, Molecular
- From:Korean Leprosy Bulletin
2010;43(1):3-13
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Appreciable amounts of high-quality DNA are frequently required for use in molecular diagnostic and epidemiology studies. The limited availability of DNA can be a critical obstacle to meeting research and clinical needs. And molecular methods have not been routinely applied to the obligate intracellular organism Mycobacterium leprae because of the difficulty of obtaining a genomic DNA template from clinical material. The MDA (multiple displacement amplification) reaction is increasingly the method of choice for many applications because of its extensive coverage of the genome, the generation of extremely long DNA products compared with older whole genome amplification methods and the high DNA yields, even from exceedingly low amounts of starting material. In the study, the author evaluated the usefulness of this method for the molecular studies in Hansen's disease.
