The effect of sodium fluoride and sodium orthovanadate on osteoblastic cell line MC3T3-E1 cells.
- Author:
Won Jin KIM
1
;
Kyu Rhim CHUNG
Author Information
1. Department of Orthodontics, College of Dentistry, Kyung Hee University, Korea.
- Publication Type:Original Article
- MeSH:
Cell Line*;
Collagen;
Collagenases;
Culture Media, Serum-Free;
DNA;
Osteoblasts*;
Proline;
Sodium Fluoride*;
Sodium*;
Thymidine;
Vanadates*
- From:Korean Journal of Orthodontics
1991;21(1):97-111
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
It is the aim of this study to investigate the effects of sodium fluoride and sodium orthovanadate upon the proliferation and activity of the osteoblast (MC3T3-E1 cells). MC3T3-E1 cells were cultured in alpha-MEM containing 10% FBS and various concentration of sodium fluoride and sodium orthovanadate was appended to serum free media. DNA synthesis was examined through the [3H] thymidine incorporation into DNA. Collagen synthesis was examined through the [3H] proline incorporation into collagenase digestible protein and noncollagen protein. The following results were drawn; 1. Sodium fluoride stimulated the DNA synthesis of osteoblast significantly in dose-dependent manner within the concentration from 2micrometer to 10micrometer (P<0.005). 2. Sodium orthovanadate stimulated the DNA synthesis of osteoblast significantly in dosedependent manner within the concentration from 2micrometer to 8micrometer, however showed diminution at 10micrometer (P<0.001). 3. Sodium fluoride and sodium orthovanadate stimulated the percent collagen synthesis of osteoblast significantly in dose-dependent manner within the concentration from 5micrometer to 10micrometer (P<0.001). 4. Sodium fluoride and sodium orthovanadate stimulated the non collagen synthesis of osteoblast significantly in dose-dependent manner within the concentration from 5micrometer to 10micrometer (P<0.001). In conclusion, sodium fluoride and sodium orthovanadate stimulate the proliferation and activity of osteoblast by stimulation of DNA synthesis and collagen and noncollagen synthesis in osteoblast.
