PCL/β-TCP Composite Scaffolds Exhibit Positive Osteogenic Differentiation with Mechanical Stimulation.
10.1007/s13770-017-0022-9
- Author:
So Hee PARK
1
;
Su A PARK
;
Yun Gyeong KANG
;
Ji Won SHIN
;
Young Shik PARK
;
Seo Rin GU
;
Yan Ru WU
;
Jie WEI
;
Jung Woog SHIN
Author Information
1. Department of Biomedical Engineering, Inje University, 197 Inje-ro, Gimhae 50834, Korea. biomechshin@gmail.com
- Publication Type:Original Article
- Keywords:
Polycaprolactone (PCL);
β-tricalcium phosphate (β-TCP);
Human mesenchymal stem cell;
3D-printing system;
Scaffold
- MeSH:
Biological Assay;
Bone and Bones;
Cell Proliferation;
Humans;
Mesenchymal Stromal Cells
- From:
Tissue Engineering and Regenerative Medicine
2017;14(4):349-358
- CountryRepublic of Korea
- Language:English
-
Abstract:
We investigated the use of Polycaprolactone (PCL)/β-tricalcium phosphate (β-TCP) composites with applied mechanical stimulation as scaffold for bone tissue engineering. PCL-based three-dimensional (3D) structures were fabricated in a solvent-free process using a 3D-printing technique. The mass fraction of β-TCP was varied in the range 0–30%, and the structure and compressive modulus of the specimens was characterized. The shape and interconnectivity of the pores was found to be satisfactory, and the compressive modulus of the specimens was comparable with that of human trabecular bone. Human mesenchymal stem cells were seeded on the composites, and various biological evaluations were performed over 9 days. With a mass fraction of β-TCP of 30%, differentiation began earlier; however, the cell proliferation rate was lower. Through the use of mechanical stimulation, however, the proliferation rate recovered, and was comparable with that of the other groups. This stimulation effect was also observed in ECM generation and other biological assays. With mechanical stimulation, expression of osteogenic markers was lower on samples with a β-TCP content of 10 wt% than without β-TCP; however, with mechanical stimulation, the sample with a β-TCP content of 30 wt% exhibited significantly greater expression of those markers than the other samples. We found that mechanical stimulation and the addition of β-TCP interacted closely, and that a mass fraction of β-TCP of 30% was particularly useful as a bone tissue scaffold when accompanied by mechanical stimulation.
