Effects of Type I Collagen Concentration in Hydrogel on the Growth and Phenotypic Expression of Rat Chondrocytes.
10.1007/s13770-017-0060-3
- Author:
Guang Zhen JIN
1
;
Hae Won KIM
Author Information
1. Institute of Tissue Regeneration Engineering (ITREN), Dankook University, 119 Dandae-ro, Dongnam-gu, Cheonan 31116, Korea. gzhjin2002@dankook.ac.kr
- Publication Type:Original Article
- Keywords:
Type I collagen;
Chondrocyte;
Proliferation;
Chondrogenic phenotype
- MeSH:
Aggrecans;
Animals;
Cartilage;
Cell Proliferation;
Cell Survival;
Chondrocytes*;
Collagen;
Collagen Type I*;
Collagen Type II;
Hydrogel*;
Hydrogels;
In Vitro Techniques;
Phenotype;
Rats*;
RNA, Messenger;
Tissue Engineering
- From:
Tissue Engineering and Regenerative Medicine
2017;14(4):383-391
- CountryRepublic of Korea
- Language:English
-
Abstract:
It is controversial whether type I collagen itself can maintain and improve chondrogenic phenotype of chondrocytes in a three-dimensional (3D) environment. In this study, we examined the effect of type I collagen concentration in hydrogel (0.5, 1, and 2 mg/ml) on the growth and phenotype expression of rat chondrocytes in vitro. All collagen hydrogels showed substantial contractions during culture, in a concentration-dependent manner, which was due to the cell proliferation. The cell viability was shown to be the highest in 2 mg/ml collagen gel. The mRNA expression of chondrogenic phenotypes, including SOX9, type II collagen, and aggrecan, was significantly up-regulated, particularly in 1 mg/ml collagen gel. Furthermore, the production of type II collagen and glycosaminoglycan (GAG) content was also enhanced. The results suggest that type I collagen hydrogel is not detrimental to, but may be useful for, the chondrocyte culture for cartilage tissue engineering.
