Role of Mast Cells in a Aspergillus Murine Model of Allergic Rhinitis.
- Author:
Jun Myung KANG
1
;
Jin Hee CHO
;
Choong Il BANG
;
Chan Soon PARK
;
Ji Hyeon SHIN
;
Dong Ho LEE
;
Heung Youp LEE
Author Information
1. Department of Otolaryngology-Head and Neck Surgery, The Catholic University of Korea, Bucheon, Korea. lhy96@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Mast cell;
Allergic rhinitis;
Animal model;
Aspergillus
- MeSH:
Animals;
Aspergillus*;
Eosinophils;
Immunoglobulin E;
Immunoglobulin G;
Immunoglobulins;
Inflammation;
Interleukin-5;
Interleukins;
Mast Cells*;
Mice;
Models, Animal;
Nasal Lavage Fluid;
Nasal Mucosa;
Rhinitis*
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2006;49(9):912-967
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: Mast cells are the main effector cells in the immediate phase of the allergic responses. To evaluate the role of mast cells on the development of allergen-induced eosinophilic inflammation in a murine model of allergic rhinitis(AR), we compared the responses of mast cell deficient (W/Wv) mice with congenial normal (W/W+) mice. MATERIALS AND METHOD: Mice were sensitized intranasally with an extract of Aspergillus fumigatue (Af) for 3 weeks, and challenged intranasally with a single dose (acute model) or single dose every day for 5 consecutive days (chronic model). Interleukin (IL)-5 in nasal lavage fluids (NLF), histologic examination and Af-specific immunoglobulins (Ig) were examined in W/Wv mice and W/W+mice. RESULTS: In the sensitized W/Wv mice, the number of eosinophils in the nasal mucosa and IL-5 in NLF after repeated allergen challenge were not significantly different from those of W/W+ mice. In contrast, the number of eosinophils in the nasal mucosa and IL-5 in NLF after single allergen challenge were partially attenuated in W/Wv mice. The levels of Af-specific IgE and IgG1 in both acute and chronic models were not different between W/Wv mice and W/W+ mice. CONCLUSION: These findings suggest that eosinophilic inflammation caused by repeated allergen challenge are independent of mast cells in this AR model.
