Determination of all Escherichia coli by multiplex PCR
- Author:
Nguyen Binh Minh
- Publication Type:Journal Article
- Keywords:
Escherichia coli
- MeSH:
Escherichia coli;
Diagnosis;
Polymerase Chain Reaction
- From:Journal of Vietnamese Medicine
2004;300(7):1-4
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
A total of 200 pathogenic strains of Escherichia coli collected on diarrhoea patients at the hospital BachMai, DongDa and DongAnh in Hanoi were studied.By multiplex PCR assay, genes of various toxicity levels were determined on 5 pathogenic E coli: 2 EPEC, 5 ETEC , 8 EAEC. There were 15 strains in which, pathogenic genes of E.coli causing diarrhoea which accounted for 7,5%.In comparing the multiplex PCR technique and simplex PCR technique, results were the same