Neurotoxic Effect of Streptozotocin and Neuroprotective Effect of Insulin Growth Factor-II to the Cultured Mouse Schwann Cells.
- Author:
Chul Ho JANG
1
;
Seung Taeck PARK
Author Information
1. Department of Otolaryngology, Wonkwang Medical School, Iksan, Korea.
- Publication Type:Original Article
- Keywords:
Cultured Schwann cells;
Streptozotocin;
IGF-II
- MeSH:
Animals;
Cell Survival;
Hearing Loss, Sensorineural;
Insulin*;
Insulin-Like Growth Factor II;
Mice*;
Nerve Growth Factors;
Neuroprotective Agents*;
Schwann Cells*;
Streptozocin*
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2000;43(6):577-581
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The sensorineural hearing loss due to diabetes is progressive and bilateral, and predominantly occurs in the old, although its accurate pathogenesis is still unknown. Objectives: The purpose of this study is to clarify the neurotoxic effect of streptozotocin (STZ) and the neuroprotective effect of insulin-like growth factor-II(IGF-II) on the cultured Schwann cells of cohlear nerve. MATERIALS AND METHODS: MTT assays were performed on cultured mouse Schwann cells which were treated with various concentrations of STZ for 24 hours, and the neuroprotective effect of IGF-II against STZ-induced neurotoxicity were also examined. RESULTS: 1) MTT50 value was the concentration of 40 pM STZ (highly toxic : MTT50<100 pM), 2) Cell viability of cultured mouse Schwann cells treated with STZ markedly decreased in a dose-dependent manner. CONCLUSION: It is suggested that STZ induces a severe toxic effect on cultured Schwann cells of mouse, and selective neurotrophic factors such as IGF-II are very effective in preventing the neurotoxicity induced by STZ.
